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In vivo Real-time assessment of anastomotic blood supply of colorectal surgery using confocal laser endomicroscopy in an anastomotic model

Xiumin Liu, Yuanzhu Liuzhang, Kai Li, Haibin Lin, Wei Jiang, Weisheng Chen, Jun Yan, MD. Department of Surgery, Guangzhou Nanfang Hospital

INTRODUCTION: Anastomotic leakage (AL) is one of the serious postoperative complications in the colorectal surgery. One of the significant factors leading to leakage is the poor bowel perfusion. Confocal laser endomicroscopy (CLE) can provide real-time observation of the cell structure and tissue morphology. In our study,we aim to assess the situation of anastomotic perfusion using CLE.

METHOD: The experimental rabbits were separated into two groups: group A (good anastomotic perfusion, n=6), group B (poor anastomotic perfusion, n=6). The partial colectomy and anastomosis was performed for group A and B. Then detection for anastomotic perfusion using CLE was carried out after the surgery. During the continuous scanning, we counted the number of blood cells that cross over the certain point of anastomotic stoma in the same period.

RESULTS: Assistant with fluorescein sodium, the blood vessels are highlighted. We can see significant difference of imaging effect between group A and group B. The average number of blood cells are 34.7/min of group A and 6.0/min of group B (p<0.001), which has significant difference.

CONCLUSION: CLE can allow real-time observation of the blood flow of anastomotic stoma in vivo. Therefore, it is feasible to assess the anastomotic perfusion using CLE in colorectal surgery.

 


Presented at the SAGES 2017 Annual Meeting in Houston, TX.

Abstract ID: 87529

Program Number: P230

Presentation Session: iPoster Session (Non CME)

Presentation Type: Poster

13

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