I. Fedoriv, MD1, I. Garcia-Alonso, MD, PHD2, B. Herrero De La Parte, PHD2, J. Guitierrez-Saenz2. 1Dpt. of Surgery. Ivano-Frankivsk national medical university. Ukraine., 2Laboratory of Experimental Surgery. Dpt. of Surgery. University of The Basque Country. Spain
Introduction: Selective portal branch ligation is being proposed as a useful technique to increase remnant liver following liver metastases surgical removal. It would be useful to check drugs which can enhance liver regeneration in this scenario without increasing tumor growth. We have developed a murine model to carry out these kind of experiments.
Methods: Three months old male WAG/RijHsd rats were randomly allocated into three groups of 7 animals each. The first group was used as control to obtain normal values of liver lobes volume and weight. The other two groups were subjected to selective ligation of the portal branch irrigating the left lateral liver lobe, and the animals were sacrificed after 3 (group PVL-3D) or 7 days (group PVL-7D). When the animals were sacrificed blood samples were obtained and the whole liver was carefully removed. The volume and weight of the whole liver and of the left lateral lobe were assessed.
Results: The left lateral lobe volume and weight was 40% of the whole liver in the control group; while it dropped to 16% three days after portal ligation, and 12% when assessed a week later. The total liver weight remained constant following portal branch ligation: Control 8.7±1, PVL-3D 7.3±1.1, PVL-7D 7.4±0.9; p=0.85).
Conclusion: Ligation of the portal branch irrigating the left lateral liver lobe, reduced the relative volume & weight of the left lateral lobe of the liver, while not affecting total liver mass.
Presented at the SAGES 2017 Annual Meeting in Houston, TX.
Abstract ID: 93222
Program Number: P325
Presentation Session: Poster Session (Non CME)
Presentation Type: Poster