Babak Banan1, Yan Wei2, Ornella Simo1, Patrick Tso3, Naji N Abumrad1, Charles Flynn1, Sinju Sundaresan1, Vance L Albaugh1. 1Vanderbilt University Medical Center, 2Beijing Shijitan Hospital, 3University of Cincinnati School of Medicine
Gastrointestinal anatomy and physiology are significantly altered by bariatric surgery, though the effects of these operations on the intestine are intrinsically difficult to study. To circumvent this challenge, we have optimized a technique for cannulation of mesenteric lymph duct (MLD) in mice as a means to study gastrointestinal physiology and endocrinology. Mice have low rates of intestinal lymph production through MLDs that are closely associated with fragile intestinal vasculature. Previous protocols for lymph collection based on the open lymph fistula model are associated with inherently low success rates in mice. With this novel approach, cannulation of the MLD using a needled-catheter maintains lymph vessel integrity, prevents lymph leakage, and is less traumatic leading to high success rates even in mice that have undergone complex bariatric surgical operations (>95%). Intestinal lymph collection in mice enables a precise study of how intestinal rearrangement effects intestinal physiology, as well as the impact of genetic manipulations on nutrient and hormone metabolism prior to their delivery into the systemic circulation.
Presented at the SAGES 2017 Annual Meeting in Houston, TX.
Abstract ID: 93482
Program Number: P612
Presentation Session: Poster Session (Non CME)
Presentation Type: Poster