Fibrin Sealant (Tissucol) Enhances Tissue Integration of cPTFE Meshes and Reduces Early Adhesion Formation in Experimental IPOM Repair

Abstract
Background: The laparoscopic intraabdominal peritoneal onlay mesh repair (IPOM) is a common technique for the reinforcement of multiple ventral hernias or defined defects after laparotomies. However, the placement of synthetic meshes in the intraabdominal cavity can be associated with severe complications. Adhesions frequently originate from the implant and protruding parts of fixation devices, presenting a serious clinical problem with potentially detrimental consequences. This study was designed to assess the impact of fibrin sealing with Tissucol® (FS; Baxter, Vienna, Austria) on adhesion formation to cPTFE (condensed polytetrafluoroethylene) meshes (Motif®Meshes, MM, Proxy Biomedical, Ireland) as well as on tissue integration of these implants in experimental IPOM repair in rats. It was tested if FS application allowed the reduction of sutures for mesh fixation without increasing the risk of mesh dislocation. Materials and methods 16 rats were assigned to the implantation of MM with 4 non resorbable sutures (Synthofil, Ethicon, Germany) with additional fibrin coating with 0.2 ml of FS or to MM fixation with 6 non resorbable sutures without FS (n=8 per group). MM with 2cm in diameter were implanted in open IPOM by a laparatomy. The observation period of 17 days ensured assessment of adhesions after the full degradation of FS. Adhesions were rated with the score suggested by Vandendael. Histology was performed. Results All 8 MMs without FS sealing elicited severe (grade III) adhesions, whereas fibrin sealed MM were rated mild in 1, moderate in 5 and severe in 2 cases. The superior finding in the FS group was statistically significant. Impaired integration of sutured-only MM was observed in 4 cases, whereas all FS sealed MM were well integrated. Conclusions FS improves the tissue integration, reduces early adhesion formation to cPTFE implants and allows reduction of perforating fixation devices in experimental IPOM repair.
Key words: IPOM; Fibrin Sealant; Tissue integration; cPTFE

Session: Poster

Program Number: P341

View Poster