• Skip to primary navigation
  • Skip to main content
  • Skip to primary sidebar
  • Skip to footer

SAGES

Reimagining surgical care for a healthier world

  • Home
    • COVID-19 Annoucements
    • Search
    • SAGES Home
    • SAGES Foundation Home
  • About
    • Who is SAGES?
    • SAGES Mission Statement
    • Strategic Plan, 2020-2023
    • Committees
      • Request to Join a SAGES Committee
      • SAGES Board of Governors
      • Officers and Representatives of the Society
      • Committee Chairs and Co-Chairs
      • Full Committee Rosters
      • SAGES Past Presidents
    • Donate to the SAGES Foundation
    • Awards
      • George Berci Award
      • Pioneer in Surgical Endoscopy
      • Excellence In Clinical Care
      • International Ambassador
      • IRCAD Visiting Fellowship
      • Social Justice and Health Equity
      • Excellence in Community Surgery
      • Distinguished Service
      • Early Career Researcher
      • Researcher in Training
      • Jeff Ponsky Master Educator
      • Excellence in Medical Leadership
      • Barbara Berci Memorial Award
      • Brandeis Scholarship
      • Advocacy Summit
      • RAFT Annual Meeting Abstract Contest and Awards
  • Meetings
    • 2022 NBT Innovation Weekend
    • SAGES Annual Meeting
      • 2023 Scientific Session Call For Abstracts
      • 2023 Emerging Technology Call For Abstracts
    • SAGES 2021 Annual Meeting
    • CME Claim Form
    • Industry
      • Advertising Opportunities
      • Exhibit Opportunities
      • Sponsorship Opportunities
    • Future Meetings
    • Past Meetings
      • SAGES 2021
      • SAGES 2020
      • SAGES 2019
      • SAGES 2018
    • Related Meetings Calendar
  • Join SAGES!
    • Membership Benefits
    • Membership Applications
      • Active Membership
      • Affiliate Membership
      • Associate Active Membership
      • Candidate Membership
      • International Membership
      • Medical Student Membership
    • Member News
      • Member Spotlight
      • Give the Gift of SAGES Membership
  • Patients
    • Healthy Sooner – Patient Information for Minimally Invasive Surgery
    • Patient Information Brochures
    • Choosing Wisely – An Initiative of the ABIM Foundation
    • All in the Recovery: Colorectal Cancer Alliance
    • Find a SAGES Member
  • Publications
    • SAGES Stories Podcast
    • SAGES Clinical / Practice / Training Guidelines, Statements, and Standards of Practice
    • Patient Information Brochures
    • TAVAC – Technology and Value Assessments
    • Surgical Endoscopy and Other Journal Information
    • SAGES Manuals
    • SCOPE – The SAGES Newsletter
    • COVID-19 Annoucements
    • Troubleshooting Guides
  • Education
    • OpiVoid.org
    • SAGES.TV Video Library
    • Safe Cholecystectomy Program
      • Safe Cholecystectomy Didactic Modules
    • Masters Program
      • SAGES Facebook Program Collaboratives
      • Acute Care Surgery
      • Bariatric
      • Biliary
      • Colorectal
      • Flexible Endoscopy (upper or lower)
      • Foregut
      • Hernia
      • Robotics
    • Educational Opportunities
    • HPB/Solid Organ Program
    • Courses for Residents
      • Advanced Courses
      • Basic Courses
    • Video Based Assessments (VBA)
    • Robotics Fellows Course
    • MIS Fellows Course
    • Facebook Livestreams
    • Free Webinars For Residents
    • SMART Enhanced Recovery Program
    • SAGES OR SAFETY Video
    • SAGES at Cine-Med
      • SAGES Top 21 MIS Procedures
      • SAGES Pearls
      • SAGES Flexible Endoscopy 101
      • SAGES Tips & Tricks of the Top 21
  • Opportunities
    • SAGES Fellowship Certification for Advanced GI MIS and Comprehensive Flexible Endoscopy
    • Foregut Fellowship Certification
    • SAGES Research Opportunities
    • Fundamentals of Laparoscopic Surgery
    • Fundamentals of Endoscopic Surgery
    • Fundamental Use of Surgical Energy
    • Job Board
    • SAGES Go Global: Global Affairs and Humanitarian Efforts
  • Search
    • Search All SAGES Content
    • Search SAGES Guidelines
    • Search the Video Library
    • Search the Image Library
    • Search the Abstracts Archive
  • Store
    • SAGES Logo Products
    • “Unofficial” Logo Products
  • Log In

The influence of pH in proximal small bowel perfusion on 64Cu absorption in anaesthetised rats

Paul Tasker, FRCS, Harbans Sharma, MSc, PhD, DSc, Joan Braganza, DSc, FRCP, Maynard Case, BSc, PhD. Manchester Royal Infirmary.

INTRODUCTION: In the closed duodenal-jejunal loop, single shot doses of standard pH solutions had a very transitory effect on duodenal luminal pH.

METHOD: To produce a more persistent change in luminal pH at different levels, the duodenal-jejunal loop with the common duct still ligated was opened and two methods of fixing pH in the gut perfusion were investigated.

RESULTS: The first method was a pH-stat re-circulation technique using single rats. As an index of 64Cu absorption, 64Cu retention in liver, as a percentage of the input concentration, from unbuffered pH stat perfusion was 36.61 (±5.61)% (n=6) (% body count per g perfusate count) at pH 6 and 22.98 (±3.09)% (n=4) at pH 8 (Mean ±SD). The second method was a triple rat buffered single pass system providing perfusion in a steady state. Four levels of pH were studied, and the same trend was observed. From a MOPS, MES and HEPES (MMH) buffer with glucose liver 64Cu retention was 33.95 (±7.84)% (n=7) at pH5, 28.02 (±5.02)% (n=3) at pH 6, 20.54 (±3.65)% (n=5) at pH7, and 16.43 (±3.60) (n=7) at pH8. Carcass and total body absorption was also measured. Then using MMH buffer without glucose, 64Cu retention in the liver only was assessed: 22.58 (± 4.53)%(n=4) at pH 5, 19.08 (±4.03)%(n=5), at pH 6, 13.84 (±3.89)%(n=4) at pH7, and 10.73 (±2.95)%(n=6) at pH 8.

As gut length varies considerably even after taking a set length (17 cm), the stretched length and mucosal weight of gut was assessed at autopsy. As independent variables their sum was divided into the percentage absorption to give gut-related absorption. For method one total 64Cu absorption was 4.21 (±0.42) (% body count per g perfusate count per g (mucosa) plus cm (length of gut loop) (n=5) at pH6 and 2.75 (±0.32) (n=4) at pH 8 (P2 <0.001). 5.5% of the gut uptake at pH 6 and 4.1% at pH 8 absorbed leaving 95% in the gut mucosa. For method two total 64Cu absorption was 4.58 (±1.72) (n=6) at pH5, 4.15 (±0.16) (n=2) at pH6, 2.33 (±0.45) (n=4) at pH 7 and 2.32 (± 0.43) (n=7) at pH8 (P2<0.001). With total absorption a jump between pH 6 and pH7 was observed which might relate to the properties of the Cu(II). 3% of 64Cu uptake absorbed leaving 97% in the gut mucosa. Net effective absorption (mucosa to body transfer) was thus a rate-limiting process.

In the pH stat recirculation study, the ratio of mucosa over the initial perfusate specific activities (counts/g) was 6.5 at pH 6 and 5.5 at pH 8. In the single pass study, the ratio was 15.8 (±5.4), at pH 5, 11.5 (± 1.1), at pH 6, 6.1 (±2.2), at pH 7 and 8.8 (±8.3), at pH 8. The specific activity ratio was greater than five to 16 times the perfusate concentration depending on pH and gut preparation.

CONCLUSION: The pH effect had its main influence at the brush-border membrane or adjacent protein binding sites.  The more acid the pH the more copper was absorbed.

 

View Poster

46

Share this:

  • Twitter
  • Facebook
  • LinkedIn
  • Pinterest
  • WhatsApp
  • Reddit

Related

« Return to SAGES 2014 abstract archive

Our Mission

Innovate, educate and collaborate to improve patient care.

Recently, on SAGES…

Surgery is Safer with Vaccination 1

Addressing Religious Concerns About COVID-19 Vaccine

This may be a difficult subject matter for you and your patient to talk about.  Be assured, all major organized religious groups encourage and recommend the COVID-19 vaccine. Listed below are references and websites you can direct your patient towards to help them make an informed decision with regards to their religious concerns against the […]

SAGES Statement on AAPI Violence

The Society of American Gastrointestinal and Endoscopic Surgeons (SAGES) stands in solidarity with the Asian American and Pacific Islander (AAPI) community. In the summer of 2020, SAGES released a statement condemning the violence, racism, and hatred toward the Black community in the wake of George Floyd and Breonna Taylor’s murders. It is with great sorrow […]

Free SAGES Webinar: Lessons from COVID on Living and Thriving as Surgeons

SAGES recognizes that the COVID-19 pandemic has had a big impact on surgical practice and in surgeon wellness. SAGES’ Reimagining the Practice of Surgery Taskforce will present “Finding the Opportunities: Lessons from COVID and How We Live and Thrive as Surgeons”  to look at ways in which innovative leadership at various levels may help transform […]

Contact SAGES

Society of American Gastrointestinal and Endoscopic Surgeons
11300 W. Olympic Blvd Suite 600
Los Angeles, CA 90064 USA
[email protected]
Tel: (310) 437-0544

Find Us Around the Web!

  • Facebook
  • Twitter
  • YouTube

Important Links

SAGES 2022 Meeting Information

Healthy Sooner: Patient Information

SAGES Guidelines, Statements, & Standards of Practice

SAGES Manuals

 

  • taTME Study Info
  • Foundation
  • SAGES.TV
  • MyCME
  • Educational Activities

Copyright © 2022 Society of American Gastrointestinal and Endoscopic Surgeons