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The Effect of Colectomy on Pancreatic Infections in a Model of Experimentally Induced Acute Pancreatitis.

Introduction; Prevention and treatment of secondary pancreatic infection is currently the main goal of treatment for acute necrotizing pancreatitis. The colon has been considered a major source of bacteria causing infection of pancreatic necrosis in acute pancreatitis. Our aim is to study the role of near total colectomy in reducing bacterial translocation and infection of pancreatic necrosis. Methods and procedures; 42 Spraque-Dawley rats were used in this study. Pancreatic ductal infusion of 1ml/kg Na-taurocholic acid was used for experimental pancreatitis. Study design was as follows; group-1; underwent laparotomy and ductal infusion of saline (control), group-2; laparotomy and ductal Na-taurocholic acid, group-3; near total colectomy and ductal saline, group-4; near total colectomy and Na-taurocholic acid. All animals were sacrificed at 48 hours, and tissue samples were collected from mesenteric lymph nodes, pancreas, spleen, liver and peritoneum for bacteriologic cultures. Additionally, blood and small bowel cultures were also prepared. Results; In group-4, small bowel bacterial overgrowth with gram-negatives microorganism such as E.coli, Proteus mirabilis and Enterococcus was observed. Bacterial count of gram-negative rods in the small intestine and pancreatic tissue were also significantly higher in group-4 than group-2 (p=


Session: Poster

Program Number: P422

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