• Skip to primary navigation
  • Skip to content
  • Skip to primary sidebar
  • Skip to footer

SAGES

Society of American Gastrointestinal and Endoscopic Surgeons

  • Home
    • Search
    • SAGES Home
    • SAGES Foundation Home
  • About
    • Committees
      • Descriptions and Video Updates
      • Request to Join a SAGES Committee
      • SAGES Board of Governors
      • Officers and Representatives of the Society
      • Committee Chairs and Mentors
      • Full Committee Rosters
      • SAGES Past Presidents
    • Donate to the SAGES Foundation
    • SAGES Mission Statement
    • SAGES Resource Guide
    • Awards
      • George Berci Award
      • Pioneer in Surgical Endoscopy
      • Excellence In Clinical Care
      • International Ambassador
      • IRCAD Visiting Fellowship
      • Distinguished Service
      • Young Researcher
      • Researcher in Training
      • Jeff Ponsky Master Educator
      • Excellence in Medical Leadership
      • The Brandeis
      • Advocacy Summit Award
      • RAFT Annual Meeting Abstract Contest and Awards
  • Meetings
    • SAGES 2019 Meeting Information
    • 2019 Scientific Session Call For Abstracts
    • 2019 Emerging Technology Session Call For Abstracts
    • 2018 Prevent BDI Consensus Conference
    • CME Claim Form
    • Industry
      • Advertising Opportunities
      • Exhibit Opportunities
      • Sponsorship Opportunities
    • Future Meetings
    • Past Meetings
      • Leadership Development and Health Policy Conference Videos
      • SAGES Quality Summit Meeting
      • SAGES 2018
      • SAGES 2017
      • SAGES 2016
      • SAGES 2015
      • SAGES 2014
      • SAGES 2013
    • Related Meetings Calendar
  • Membership
    • Join SAGES!
    • Give the Gift of SAGES Membership
    • Membership Benefits
    • Active Membership
    • Allied Health Membership
    • Associate Active Membership
    • Candidate Membership
    • International Membership
    • Medical Student Membership
  • For Patients
    • Healthy Sooner – Patient Information for Minimally Invasive Surgery
    • Patient Information Brochures
    • Choosing Wisely – An Initiative of the ABIM Foundation
    • Find a SAGES Member
  • Publications
    • SAGES Clinical / Practice / Training Guidelines, Statements, and Standards of Practice
    • Patient Information Brochures
    • TAVAC – Technology and Value Assessments
    • Surgical Endoscopy and Other Journal Information
    • SAGES Manuals
    • SCOPE – The SAGES Newsletter
    • Troubleshooting Guides
  • Education
    • The Safe Cholecystectomy Didactic Modules
    • Masters Series
      • SAGES Masters Program Facebook Collaboratives
      • Acute Care Surgery
      • Bariatric
      • Biliary
      • Colorectal
      • Flexible Endoscopy (upper or lower)
      • Foregut
      • Hernia
      • Robotics
    • Educational Opportunities
    • The SAGES HPB/Solid Organ Program
    • The SAGES Safe Cholecystectomy Program
    • Courses for Residents
      • Advanced Courses
      • Basic Courses
    • Endorsed Courses
    • SAGES Robotics Fellows Course
    • MIS Fellows Course
    • Free Educational Webinars
    • SMART Enhanced Recovery Program
    • SAGES Quality Initiative
    • SAGES OR SAFETY Video
    • SAGES.TV Video Library
    • SAGES Surgical WIKI
    • iMAGES Image Library
    • SAGES at Cine-Med
      • SAGES Top 21 MIS Procedures
      • SAGES Pearls
      • SAGES Flexible Endoscopy 101
      • SAGES Tips & Tricks of the Top 21
  • Opportunities
    • SAGES Research Opportunities
    • SAGES Endorsed Courses
    • Fundamentals of Laparoscopic Surgery
    • Fundamentals of Endoscopic Surgery
    • Fundamental Use of Surgical Energy
    • Job Board
    • SAGES Go Global: Global Affairs and Humanitarian Efforts
  • Search
    • Search All SAGES Content
    • Search the Video Library
  • Log In

Real-time Optical Diagnosis for surgical margin in low rectal cancer Using Multiphoton Microscopy

Jun Yan, MD, Shuangmu Zhuo, PhD, Gang Chen, Mingang Ying, MD

Department of Surgery, Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, 350014, P.R.China; Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laborato

Background:
Multiphoton microscopy (MPM), based on the advancement of the field of non-linear optics and femtosecond lasers, has been shown to provide real-time detailed information on tissue architecture and cell morphology in live tissue. The purpose of this study was to evaluate the feasibility of using MPM to make real-time optical diagnosis for surgical margin in low rectal cancer.

Methods: Thirty fresh, unfixed, and unstained full-thickness surgical margins in low rectal cancer underwent MPM examination and then went through routine intra-operative pathological frozen procedure. MPM images were compared with golden standard hematoxylin-eosin (H-E) stained images.

Results: MPM images were obtained by two photon-excited fluorescence signals from tissue sample. Peak multiphoton autofluorescence intensity was detected in mucosa excited at 800 nm. In the normal area of surgical margin, MPM revealed regular tissue architecture and cell morphology, including a typical foveolar pattern with central, round crypt openings, and glands lined by epithelian cells and goblets cells. In the cancerous area of surgical margin, MPM demonstrated irregular tubular structures, reduced stroma, and cellular and nuclear pleomorphism. Cancer cells, characterized by irregular size and shape, enlarged nuclei, and increased nuclear-cytoplasmic ratio, were identified by MPM images, which were comparable to H-E stained images.

Conclusions: It is feasible to use MPM to make real-time optical diagnosis for surgical margin in low rectal cancer. With miniaturization and integration of colonoscopy or probe, MPM has the potential to provide real-time non-invasive “optical biopsy” for surgical margin in low rectal cancer in the near future.

Key words: Multiphoton microscopy; Real-time diagnosis; Surgical margin; Low rectal cancer; Pathology.


Session: Podium Presentation

Program Number: S043

Post Views: 16

Share this:

  • Twitter
  • Facebook
  • LinkedIn
  • Pinterest
  • WhatsApp
  • Reddit

Related

« Return to SAGES 2013 abstract archive

Recently, on SAGES…

Robotic Surgery

Spotlight: Robotics Committee

In the most recent issue of SCOPE, SAGES President Dr. Jeffrey Marks shared his desire to highlight the hard work of the many SAGES committees. As the Society strives to fully realize the new SAGES University Masters Program, the initial focus will be on the affiliated pathway committees and task forces. This is the third […]

Mastering the Art of Surgery – Annual Meeting Press Release

Mastering the Art of Surgery At the 16th World Congress of Endoscopic Surgery and 2018 SAGES Meeting April 11th–14th in Seattle, WA The Society of American Gastrointestinal and Endoscopic Surgeons (SAGES) will kick off its 2018 Annual Meeting as part of the 16th World Congress of Endoscopic Surgery. Hosted by SAGES and CAGS (Canadian Association […]

Complete the SAGES Video Based Assessment Survey for a Chance to Win!

https://survey.az1.qualtrics.com/jfe/form/SV_5pCM9JWvDBphkjj Who Can Enter Open to any surgeon who is either a member of SAGES or an attendee at the 2018 SAGES Annual Meeting AND who completes the 2018 SAGES Video Based Assessment Online Survey at the link above. Drawing Entry Period The drawing period is from survey launch on April 6, 2018 to 11:59pm […]

Contact SAGES

Society of American Gastrointestinal and Endoscopic Surgeons
11300 W. Olympic Blvd Suite 600
Los Angeles, CA 90064 USA
webmaster@sages.org
Tel: (310) 437-0544

Find Us Around the Web!

  • Facebook
  • Twitter
  • YouTube

Newsletter Subscription

  • This field is for validation purposes and should be left unchanged.

Important Links

SAGES 2019 Meeting Information

Online Registration coming in Fall 2018

Healthy Sooner: Patient Information

  • taTME Study Info
  • Foundation
  • SAGES.TV
  • MyCME
  • Educational Activities

· Log in

Copyright © 2019 Society of American Gastrointestinal and Endoscopic Surgeons · Legal
· Managed by BSC Management, Inc