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You are here: Home / Abstracts / Of Mice of Mesh: Evaluating mesh structure on bacterial adherence

Of Mice of Mesh: Evaluating mesh structure on bacterial adherence

Lawrence N Cetrulo, MD1, Alisan Fathalizadeh, MD, MPH2, Michelle Nguyen, MD2, Pak Shan Leung2. 1Carolinas Healthcare Network, 2Einstein Healthcare Network

Introduction: Prosthetic infections, although relatively uncommon, are a major source of cost and morbidity.  The study aimed to evaluate the influence of mesh structure including the polymer type and mean pore size on bacterial adherence in a mouse model.

Methods: Three commercially available hernia meshes were included in the study. For each mesh type, a 1 cm square was surgically placed intraabdominally in 6 mice.  One mouse served as a control while an enterotomy was made in the subsequent mice to introduce a bacterial load onto the mesh.  After 24 hours the meshes were harvested.  The inoculated meshes were then plated on agar plates and bacterial counts were counted after 24 hours.  The bacterial counts were compared between the various mesh types.

Results: The mean bacterial adherence was increased in the large pore mesh was 695 colonies, for the small pore mesh was 892 colonies, and in the biologic mesh group it was 504 colonies.

Conclusions: Through the use of a mouse model, the influence of mesh type and pore size on bacterial adherence was evaluated.  Meshes that have larger pores with a lower prosthetic load and the biologic mesh interestingly had lower early bacterial colonization after 24 hours following an enterotomy. Further evaluation with a longer incubation time could be helpful to determine the effect of bacterial colonization of mesh. 


Presented at the SAGES 2017 Annual Meeting in Houston, TX.

Abstract ID: 86532

Program Number: P023

Presentation Session: iPoster Session (Non CME)

Presentation Type: Poster

32

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