• Skip to primary navigation
  • Skip to main content
  • Skip to primary sidebar
  • Skip to footer

SAGES

Reimagining surgical care for a healthier world

  • Home
    • COVID-19 Annoucements
    • Search
    • SAGES Home
    • SAGES Foundation Home
  • About
    • Who is SAGES?
    • SAGES Mission Statement
    • Advocacy
    • Strategic Plan, 2020-2023
    • Committees
      • Request to Join a SAGES Committee
      • SAGES Board of Governors
      • Officers and Representatives of the Society
      • Committee Chairs and Co-Chairs
      • Full Committee Rosters
      • SAGES Past Presidents
    • Donate to the SAGES Foundation
    • Awards
      • George Berci Award
      • Pioneer in Surgical Endoscopy
      • Excellence In Clinical Care
      • International Ambassador
      • IRCAD Visiting Fellowship
      • Social Justice and Health Equity
      • Excellence in Community Surgery
      • Distinguished Service
      • Early Career Researcher
      • Researcher in Training
      • Jeff Ponsky Master Educator
      • Excellence in Medical Leadership
      • Barbara Berci Memorial Award
      • Brandeis Scholarship
      • Advocacy Summit
      • RAFT Annual Meeting Abstract Contest and Awards
  • Meetings
    • NBT Innovation Weekend
    • SAGES Annual Meeting
      • 2023 Scientific Session Call For Abstracts
      • 2023 Emerging Technology Call For Abstracts
    • CME Claim Form
    • Industry
      • Advertising Opportunities
      • Exhibit Opportunities
      • Sponsorship Opportunities
    • Future Meetings
    • Past Meetings
      • SAGES 2022
      • SAGES 2021
    • Related Meetings Calendar
  • Join SAGES!
    • Membership Benefits
    • Membership Applications
      • Active Membership
      • Affiliate Membership
      • Associate Active Membership
      • Candidate Membership
      • International Membership
      • Medical Student Membership
    • Member News
      • Member Spotlight
      • Give the Gift of SAGES Membership
  • Patients
    • Healthy Sooner – Patient Information for Minimally Invasive Surgery
    • Patient Information Brochures
    • Choosing Wisely – An Initiative of the ABIM Foundation
    • All in the Recovery: Colorectal Cancer Alliance
    • Find a SAGES Member
  • Publications
    • SAGES Stories Podcast
    • SAGES Clinical / Practice / Training Guidelines, Statements, and Standards of Practice
    • Patient Information Brochures
    • TAVAC – Technology and Value Assessments
    • Surgical Endoscopy and Other Journal Information
    • SAGES Manuals
    • SCOPE – The SAGES Newsletter
    • COVID-19 Annoucements
    • Troubleshooting Guides
  • Education
    • OpiVoid.org
    • SAGES.TV Video Library
    • Safe Cholecystectomy Program
      • Safe Cholecystectomy Didactic Modules
    • Masters Program
      • SAGES Facebook Program Collaboratives
      • Acute Care Surgery
      • Bariatric
      • Biliary
      • Colorectal
      • Flexible Endoscopy (upper or lower)
      • Foregut
      • Hernia
      • Robotics
    • Educational Opportunities
    • HPB/Solid Organ Program
    • Courses for Residents
      • Advanced Courses
      • Basic Courses
    • Video Based Assessments (VBA)
    • Robotics Fellows Course
    • MIS Fellows Course
    • Facebook Livestreams
    • Free Webinars For Residents
    • SMART Enhanced Recovery Program
    • SAGES OR SAFETY Video
    • SAGES at Cine-Med
      • SAGES Top 21 MIS Procedures
      • SAGES Pearls
      • SAGES Flexible Endoscopy 101
      • SAGES Tips & Tricks of the Top 21
  • Opportunities
    • NEW-Area of Concentrated Training Seal (ACT)-Advanced Flexible Endoscopy-Coming Soon!
    • SAGES Fellowship Certification for Advanced GI MIS and Comprehensive Flexible Endoscopy
    • Multi-Society Foregut Fellowship Certification
    • SAGES Research Opportunities
    • Fundamentals of Laparoscopic Surgery
    • Fundamentals of Endoscopic Surgery
    • Fundamental Use of Surgical Energy
    • Job Board
    • SAGES Go Global: Global Affairs and Humanitarian Efforts
  • Search
    • Search All SAGES Content
    • Search SAGES Guidelines
    • Search the Video Library
    • Search the Image Library
    • Search the Abstracts Archive
  • Store
    • “Unofficial” Logo Products
  • Log In

In Vivo Fluorescence Imaging of Staphylococcus Aureus Biologic Mesh Infection – a 30 Day Analysis

BACKGROUND: Infection in the setting of hernia repair poses a difficult challenge, and biologic mesh might perform favorably in this setting. The specific response of different biologic mesh constructs to infection is unclear. Using fluorescent-labeled bacteria and innovative in vivo imaging quantitative bacterial analysis, we evaluated the response of two biologic mesh prosthetics to staphylococcus aureus infection.

METHODS: Twenty-four rats underwent creation of a chronic hernia. They were randomly assigned to underlay bridge repair with Permacol (cross linked porcine dermis) (n=12) or Surgisis (noncrosslinked porcine submucosa) (n=12). Half in each group were repaired in sterile fashion (clean cases; n=6) and half were inoculated with 104 CFU/ml of a clinical strain of green fluorescent protein (GFP) labeledStaphylococcus aureus (SA) (clean contaminated cases; n=6). Animals were allowed to survive 30-days, euthanized and the explanted abdominal wall underwent immediate in vivo fluorescent imaging. A 6mm punch biopsy of both tissue and mesh was obtained after imaging. This was followed by homogenizing, serial diluting, plating in blood agar dishes, incubation for 24 hours at 37° C and CFU/gm estimates. Validation of our imaging was performed by in vitro fluorescence imaging of serial concentrations of GFP SA (103 – 108 CFU/ml) to determine the minimal level of GFP signal detection. Fisher’s exact statistic was performed.

RESULTS: All animals survived 30 days following repair. CLEAN CASES: Culture results for both clean groups did not reveal evidence of infection (0 CFU/gm). CLEAN CONTAMINATED CASES: One of six (17%) Permacol animals had residual GFP SA infection (8 x 102 CFU/gm; n=1) while the remainder had no GFP SA (0 CFU/gm; n=5). Three of six (50%) Surgisis animals had residual GFP SA infection (1.3 x 102 CFU/gm; n=3) while the others had no detectable GFP SA infection (p-value = 0.55). IMAGING: In vivo imaging corroborated our low bacterial counts and was unable to detect presence of GFP SA or a difference in signal between clean contaminated cases of Permacol or Surgisis (0 RFU signal/group; n=12). IN VITRO VALIDATION: There was a positive linear correlation (R2 = 0.9121; Y=715X+186) between imaging device signal and bacterial counts above a concentration of 105 CFU/ml. This was determined to be the lower limit of GFP SA detection with our in vivo fluorescence-imaging device.

CONCLUSIONS: This study confirms that biologic mesh can tolerate clean contaminated situations. In these settings most animals were able to clear the bacterial contamination as demonstrated by low quantitative cultures and corroborated with in vivo fluorescent imaging. Further analysis as to the exact mechanism of bacterial clearance and biomechanical alterations of the mesh in infected fields is ongoing.


Session: Podium Presentation

Program Number: S065

409

Share this:

  • Twitter
  • Facebook
  • LinkedIn
  • Pinterest
  • WhatsApp
  • Reddit

Related

« Return to SAGES 2010 abstract archive

Our Mission

Innovate, educate and collaborate to improve patient care.

Recently, on SAGES…

Critical View of Safety (CVS) Challenge QR Code

The SAGES Critical View of Safety Challenge – Donate Your Lap Chole Videos!

The Society of American Gastrointestinal and Endoscopic Surgeons is hosting the first Artificial Intelligence Data Challenge conducted by surgeons. The aim of this challenge is to generate a large and diverse dataset of laparoscopic cholecystectomy videos, annotated with respect to the subcomponents of the Critical View of Safety (CVS). Computer scientists from all over the […]

Respuesta de SAGES al Estudio NordICC sobre el beneficio de las colonoscopias de detección

SAGES desea aclarar los resultados del estudio NordICC y colocarlos en contexto de los esfuerzos de varias agencias nacionales para reducir el riesgo de cáncer colorrectal – la segunda causa de muerte por cáncer más frecuente en los Estados Unidos-, mediante la promoción de la detección y tratamiento oportuno de las lesiones.

SAGES Response to NordICC Study Regarding Benefit of Screening Colonoscopies

The NordICC Study recently published in The New England Journal of Medicine and widely reported on by media outlets has raised questions regarding the benefit of screening colonoscopy in lowering the risk of colorectal cancer and cancer-related deaths among otherwise healthy and symptom-free men and women aged 55 to 64. Provocative headlines and commentaries have […]

Contact SAGES

Society of American Gastrointestinal and Endoscopic Surgeons
11300 W. Olympic Blvd Suite 600
Los Angeles, CA 90064 USA
webmaster@sages.org
Tel: (310) 437-0544

Find Us Around the Web!

  • Facebook
  • Twitter
  • YouTube

Important Links

SAGES 2023 Meeting Information

Healthy Sooner: Patient Information

SAGES Guidelines, Statements, & Standards of Practice

SAGES Manuals

 

  • taTME Study Info
  • Foundation
  • SAGES.TV
  • MyCME
  • Educational Activities

Copyright © 2023 Society of American Gastrointestinal and Endoscopic Surgeons