Ebru Esen1, Siyar ErsOz2, Ibrahim Ethem Gecim3, Fikret Sahin4, Ozlem Kücük5, Mustafa Ertek6, Mustafa Filik5, Atilla Elhan7. 1ankara university medikal faculty surgical oncology department, 2Ankara Numune Reseach and Training Hospital, 3ankara university medical faculty general surgery department, 4Ankara University Medical Faculty Medical Microbiology Department, Ankara, Turkey., 5Ankara University Medical Faculty Nuclear Medicine Department, Ankara, Turkey., 6Diskapi Yildirim Beyazit Egitim Arastirma Hastanesi Klinik Mikrobiyoloji ve Enfeksiyon Hastaliklari,, 7Ankara University Medical Faculty Medical Biostatistics Department, Ankara, Turkey.
Purpose : Between laparoscopic and open surgery for tumoral organisms, after laparoscopic surgery immune system of organism supresses lesser, for this reason, by some, it is believed that after surgery the remaining tumor cells grow more slowly. The aim of this study is to investigate the effects of laparoscopic and open surgery to tumor growth and activity.
Methods : Five- to six- weeks- old female, thirty, BALB/C mice were used for this study. 2.5×106 CT- 26 tumor cells were inoculated intradermally in the dorsal skin of mice. The mice were randomised in three groups, every group included ten animals.1. group; control group, 2. group ; open surgery model, 3. group; three port laparoscopy model. After administration of anestesia to 1. group, no operation was made. After administration of anesthesia to 2. group, 3cm laparotomy was made and waited for 20 minutes then skin and fascia closed.After administration of anesthesia to 3. group, 18 gauge needle inserted to abdomen and CO2 pneumoperitoneum was created, then 2 more 18 gauge needles were inserted. For 15 minutes a pressure of 2 to 4 mmHg was maintained. At fifteenth minute 5 mm mini insicion was made, wait for 5 minutes and then skin and fascia closed. At 12th hour we have received tail vein blood and IL-6 levels were measured. At fourteenth day visible tumor nodules grew up. Long and short tumor diameters milimetrically measured. At fifteenth day, under anesthesia PET was taken. Same day all animals were sacrifised and tumors en- block resected and weighed.
Results : There was no staticallay significant difference for 3 group’s tumor weights. There was no statically significant difference in tumor long and short diameters for laparoscopic and laparotomy groups. Diameters of the two groups were statistically significantly longer than the control group. There was no statically significant difference in SUVmax values for laparoscopic and laparotomy groups. SUVmax values of the two groups were statistically significantly higher than the control group. There was no statically significant difference in IL-6 values for laparoscopic and laparotomy groups. IL-6 values of the two groups were statistically significantly higher than the control group
Conclusions : In coclusion, there was no significant difference for IL-6, SUVmax values, weight and tumor size between laparoscopy and laparotomy groups. In later periods, with larger groups and more parameters, experiments will provide clearer and more reliable results.