Changes in morphological condition of small intestine in rats after 50% distal resection of small bowel and after correction GLP-1 and GLP-2 peptides.

Ivan V Fedoriv, MD, Natalia V Sopchuk, MD, Volodymir F Knygnytskyy, MD, Yuriy I Popovych, PhD. Department of human anatomy, operative surgery and topographic anatomy IFNMU, Ivano-Frankivsk, Ukraine

Introduction: The influence of GLP-1 and GLP-2 peptides in compensatory-renewal mechanisms of small bowel after it’s 50% distal resection isn’t fully researched.

Objective: The influences of GLP-1 and GLP-2 peptides, which are produced by L-cells of ileum and colon have a trophic effect on leftover parts of small bowel and increase adaptive processes.

Methods: 5 rats for each 7, 14, 30, 90 days after resection: without curation – intact 20 rats, Glp-1 same days, so in total 20 rats, Glp-2 also 20 rats, and we were researching 3 parts of leftover small bowel (jejunum, duodenum, ileum). All operations were done under thiopental anesthesia.

Results: On the 7-th day after resection using Glp-1 we see decrease of wall thickness in duodenum (586±24,89) μm from (742±11,74) μm – norm, jejunum without serious changes, approximately, as in norm – (573±13,86) μm  and in ileum we signalize  increase of wall thickness (612±21,12) μm from (403±10,54) μm – norm.  After researching of  Glp-2 action on the 7-th day after 50% resection we can make conclusion, that the wall thickness increases in 1,3 times in duodenum and in 1,4 times in ileum, but in jejunum changes are miserable, as in comparing with norm results.

On the 14-th day after resection using GLP-1 we have an increased wall thickness in all divisions of small intestine, but the best is in ileum, more than 1.8 times as in control. And after treatment of Glp-2 the biggest wall thickness we have got in duodenum (848±16.24)  μm, but the biggest increase was in ileum – 307  μm, jejunum – 295 μm, more less in duodenum – 273 μm.

The laws of  changes what we got on 14 days is almost similar with 7 days instead of jejunum on the first 7 days we received an decrease of wall thickness even according the control, on 14 days we have got a little increasing but it was still less than in norm (decreasing both – height of villi and crypt).

Conclusion: compensatory-repairing reactions after distal resection of small bowel are better when use GLP-2, because of increasing of wall thickness in almost all parts of small intestine without abrupt falling of some exponents under using Glp-1( for example changes in duodenum at 7-th day). So, using Glp-drugs is acquitted, in postoperative period.