Minimally invasive colorectal resection for cancer but not benign disease is associated with a short lived decrease in soluble Tie-2 Receptor levels which likely promotes VEGF-mediated angiogenesis

Introduction: Plasma VEGF levels are increased after minimally invasive colorectal resection (MICR) and likely stimulate angiogenesis in the wound and in residual tumor deposits. Angiopoetin (Ang) 1, via binding to endothelial cell (EC) bound Tie-2 receptor (Tie2) inhibits VEGF mediated angiogenesis while Ang 2 encourages VEGF-related angiogenesis by preventing Ang-1’s effects via competitive binding of EC bound Tie2. Ang 2 levels are increased and Ang 1 levels decreased after MICR. Soluble Tie2, which is not capable of signal transduction, binds free Ang 1 and 2 in the blood, and modulates the impact of these proteins on angiogenesis. The purpose of this study was to assess the effect of MICR on plasma sTie2 levels.
Methods: Blood samples were obtained preoperatively (preop) and on postoperative day (POD) 1 and 3 in patients undergoing MICR for benign indications (LB, n=41) and for cancer (LC, n=34). In a subgroup of patients a 4th specimen was obtained between POD 7 and 13. The breakdown of operations performed was not statistically different. sTie2 levels were determined via ELISA and the mean and SD were determined for each parameter and time point. The POD 7-13 specimens were bundled and considered as one time point. t-test was used for analysis and significance defined as p

Session: Poster

Program Number: P125

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